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Ribo-TISH

note

Identifies translated ORFs from Ribo-seq data and reports reading frame quality metrics.

https://github.com/zhpn1024/ribotish

Ribo-TISH is a tool for identifying translated ORFs from Ribo-seq data. This module parses the *_qual.txt output files to visualize reading frame quality metrics across different read lengths.

The module creates one of two visualizations:

  1. A stacked bar chart showing the proportion of reads in each reading frame (Frame 0, 1, 2) for read lengths 25-34nt
  2. A heatmap showing the percentage distribution of read lengths within each sample

File search patterns​

ribotish/qual:
  fn: '*_qual.txt'
  num_lines: 10